Effect of Moringa oleifera leaves extract on biochemical, hematological and histopathological parameters in Trypanosoma brucei infected rats

Galadima, Abdulganiyu M.; O., Grace Ezimaduakolam; Abubakar, Ibrahim; Said, Said S.; Umar, Sanusi F.

doi:10.21608/bbj.2025.350281.1072

Effect of Moringa oleifera leaves extract on biochemical, hematological and histopathological parameters in Trypanosoma brucei infected rats

Document Type : Research Articles

Authors

¹ Department of Biochemistry, School of Biological Sciences, Federal University of Technology, Owerri, Imo State, Nigeria

² Department of Biology-Chemistry, Idris Koko Technical College, Farfaru, Sokoto, Nigeria

³ Department of Biochemistry and Molecular Biology, Faculty of Life Science, Federal University, Dutsin-Ma, Katsina, Nigeria

10.21608/bbj.2025.350281.1072

Abstract

Trypanosomiasis remains an endemic disease in several societies affecting human and animals, causing a high mortality rate. Moringa oleifera has been used traditionally in the treatment of many diseases, including infections, cardiovascular diseases, hypertension, and cancers. This study aims to evaluate the effect of M. oleifera leaves extract (MOLE) on biochemical, hematological, and histopathological parameters in Trypanosoma brucei infected rats. The trypanosoma infection was induced in rats using the rapid matching method. Hematological parameters were analyzed using a standard hematology analyzer. Biochemical parameters were analyzed by ELISA and spectrophotometric methods. MOLE with concentrations of 400 and 800 mg/kg significantly (p< 0.05) reduced parasitemia level in the infected rats. The RBC, PVC, Hb, WBC, PLT, MCV, MCH, and MCHC contents were significantly (p< 0.05) increased in the infected treated rats. Treatment of the infected rats significantly (p< 0.05) decreased the level of ALT, AST, ALP, total bilirubin, urea, creatinine, total cholesterol, TAG, LDL-C, and VLDL-C. However, a significant (p< 0.05) increase in total protein, albumin, globulin, HDL-C, sodium, potassium, and chloride levels was observed in the infected treated rats. The adverse changes in the liver and kidney tissues of the infected rats were significantly (p< 0.05) reversed to normal hepatocytes and normal components of the portal triads in the treated rats. MOLE demonstrated anti-trypanosomal activity and diminished the adverse alterations in the biochemical, hematological and histopathological parameters induced by T. brucei in the rats.

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