A Possible effect of Moringa oleifera seed oil or/and with praziquantel in ameliorating liver damage in Shistosoma mansoni infected adult male albino mice

Khalaf, Fatema M.; Osman, Gamalat Y.; Mohamed, Azza H.; Yassien, Rania I.; Elmalawany, Alshimaa M.

doi:10.21608/bbj.2025.355354.1079

A Possible effect of Moringa oleifera seed oil or/and with praziquantel in ameliorating liver damage in Shistosoma mansoni infected adult male albino mice

Document Type : Original Article

Authors

¹ Zoology Department, Faculty of Science, Menoufia University, Shebin El Kom, Egypt

² Histology Department, Faculty of Medicine, Menoufia University, Shebin El Kom, Egypt

³ Clinical Pathology Department, National Liver Institute, Menoufia University, Shebin El Kom, Egyptpt

10.21608/bbj.2025.355354.1079

Abstract

Schistosomiasis affects persistly the health and quality of life of millions of people. World Health Organization (WHO) approve only praziquantel (PZQ) drug for schistosomiasis treatment. Parasites have developed resistance to this drug. Thus, searching for new alternatives is necessary. This study assessed the effectiveness of Moringa oleifera seed oil alone or combined with PZQ drug targeting–infected mice with Schistosoma mansoni-induced liver damage. Mice were divided into five groups, group (G) I: control, G II: Infected mice, G III: Mice Infected and treated with PZQ, and G IV: Mice infected and treated with M. oleifera seed oil. G V: Mice infected and treated with PZQ accompanied with M. oleifera seed oil. Liver section obtained from S. mansoni infected mice displayed granuloma which consisted of Schistosoma egg embracing by a massive inflammatory cells and fibrotic tissues. Atypical hepatocytes manifested pyknotic nuclei and excessive lymphocytic infiltration PZQ infected group and M. oleifera seed oil infected group showed apparent decreased granuloma diameters. Infected mice treated with M. oleifera seed oil and PZQ together notably diminished the worms number in tissue. In addition, the liver showed less change in histopathology and less significant exhibition of IgG, glutathione S transferrase, catalase and intercellular adhesion molecules, ICAM. Combination of M. oleifera seed oil extract with PZQ has a therapeutic effect on S. mansoni infection and helps reduce the effects of damage.

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