Document Type : Original Article
Authors
1
Biotechnology Program, Faculty of Agriculture, Ain Shams، Cairo, Egypt
2
Biotechnology Program, Faculty of Science, Helwan University, Cairo, Egypt
3
Biotechnology program, Faculty of Science, Tanta University, Tanta, Egypt
4
Biotechnology Program, Faculty of Science, Ain Shams University, Cairo, Egypt
Abstract
Monkeypox (Mpox) virus has been a neglected tropical zoonotic disease for more than 50 years. Due to its rapidly spreading outbreak in more than 100 countries and territories, recently, becomes a focus of global attention. It is a contagious zoonotic disease caused by the Orthopoxvirus, endemic in Central and West Africa, and has symptoms similar to that caused by smallpox viruses. Symptoms of Mpox typically include fever, chills, rash, and lesions on the genitals or face. Hundreds of human clinical samples have undergone next-generation sequencing (NGS), with raw data deposited in public repositories, since the global outbreak in 2022. However, sequence analysis for in-depth investigation of virus evolution is still hampered by the lack of a complete protein database for the Mpox virus. Viral protein sequence data are essential for scientific prevention and epidemic management. Although many databases include protein structures, there is a significant gap in viral protein structures. To address this critical gap, the Mpox protein (MOPO) database was developed, which integrates 180 predicted 3D protein structures of the Mpox virus. Using the AlphaFold server, we predicted and validated protein structures with high template modeling scores (pTM). This database serves as a repository for all predicted protein isoforms of the Mpox virus. All predicted protein structures were uploaded to the database, and it became possible for any researcher or person interested in the field of viruses to obtain the structure of any protein, which is a quick and useful way for researchers to enhance clinical research for infected patients.
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