Musa paradisiaca leaves extract ameliorates cyclophosphamide-induced hepato-renal toxicity in mice

Authors

1 Nutrition and Food Science, Faculty of Specific Education, Tanta University Egypt

2 Anatomy &Embryology Department, Faculty of Medicine, Tanta University, Egypt

3 Zoology Department, Faculty of Science, Tanta University, Egypt

Abstract

Chemotherapeutic agents, cyclophosphamide (CTX) is accompanied by severe
adverse effects on multi-vital organs. Leaves and peels of Musa trees showed
promising biomedical applications. The study aims to assess the ameliorative
effects of Musa leaves (MLE) and Musa peels (MPE) extracts on the hepatorenal dysfunctions caused by CTX in mice. The acute median lethal dose (LD50)
was determined using forty mice (CD-1). Other forty male CD-1 mice were
divided into four groups (n=10) as follows: Group1 (Gp1) had injected
intraperitoneally (i.p.) with 200 µl of sterile saline. Gp2 had injected i.p. with a
single dose of CTX as 200 mg/kg b.wt. Gp3 and Gp4 were injected with CTX
as in Gp2 then injected with 1/20 LD50 of MLE (175 mg/kg) or MPE (170
mg/kg) daily, respectively. Fifty percent (50%) of mice in all groups were
sacrificed after 15 days of treatments, and the rest of mice were sacrificed by
day 30. All groups were bled at days 3, 9, 15, 23 and 30 to determine the
hematological changes. Biochemical parameters, liver, and kidney histological
changes were assessed at days 15 and 30 of treatment. The LD50 of MLE and
MPE were 3500 and 3400 mg/kg, respectively. CTX injection led to a
significant decrease in white blood cells (WBCs) count starting from day 3 to
day 12 (leucopenic phase), increased liver enzymes (AST and ALT) activities,
induced liver, and kidney histological alterations. Treatment with MLE post
CTX partially restored the total WBCs count in the leucopenic phase. Further,
MLE treatment improved the biochemical and histopathological changes as
evidenced by restoring liver, kidney functions and improving their histological
architectures in CTX-injected mice. Collectively, MLE treatment could protect
the hepato-renal tissues from toxicities which were induced by CTX.

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